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rat monoclonal anti mcherry  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rat monoclonal anti mcherry
    Rat Monoclonal Anti Mcherry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mcherry/Saponin/pm40866713-622-28-42
    Average 99 stars, based on 1 article reviews
    rat monoclonal anti mcherry - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Cells and circuits for amygdala neuroplasticity in the transition to chronic pain.
    Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween 20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. The slices were incubated in rat anti-mCherry (1:125, M11217, Invitrogen) and rabbit anti-b-gal (1:500, ab986, Millipore Sigma) primary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA overnight at 4 C. Slices were then rinsed in PBS and incubated in goat anti-rat Cy3 (1:250, A10522, Invitrogen) and Alexa Fluor 647-conjugated goat anti-rabbit (1:500, A21244, Invitrogen) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 h at room temperature. .. Sections were then rinsed in PBS, mounted on positively charged glass slides, air-dried and coverslips were placed using Fluoromount-G (Southern Biotech).

    Article Title: Social transmission of valence-linked new knowledge without firsthand experience in mice
    Article Snippet: Brains were removed and post-fixed in 4 % PFA in PBS at 4 ° C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm. .. For immunohistochemistry (IHC), tissue sections were incubated in 0.3 % Triton-X/PBS (PBS-T) with 10 % normal goat serum (NGS) for 1 h. Primary antibodies were then added to PBS-T with 10 % NGS and tissue sections were incubated overnight at 4 ° C. Primary antibodies used for immunostaining were as follows: guinea pig anti-NeuN (1:1000, Millipore Sigma), rabbit anti-GFP (1:2000, Thermo Fisher Scientific) and rat anti-mCherry (1:2000, Invitrogen). .. After washing with PBS (3 x 5 min), tissue sections were subsequently incubated with AlexaFluor 488, AlexaFluor 546, or AlexaFluor 555 conjugated secondary antibodies (1/500, Thermo Fisher Scientific) in PBS with 10 % NGS for 2 h at R.T. Tissue sections were then incubated in PBS-T with DAPI (1:1000, Thermo Fisher Scientific) for 20 min, and then washed with PBS-T (3 x 10 min) at R.T., and mounted in VECTASHIELD medium (Vector Laboratories) on glass slides.

    Next-Generation Sequencing:

    Article Title: Cells and circuits for amygdala neuroplasticity in the transition to chronic pain.
    Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween 20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. The slices were incubated in rat anti-mCherry (1:125, M11217, Invitrogen) and rabbit anti-b-gal (1:500, ab986, Millipore Sigma) primary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA overnight at 4 C. Slices were then rinsed in PBS and incubated in goat anti-rat Cy3 (1:250, A10522, Invitrogen) and Alexa Fluor 647-conjugated goat anti-rabbit (1:500, A21244, Invitrogen) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 h at room temperature. .. Sections were then rinsed in PBS, mounted on positively charged glass slides, air-dried and coverslips were placed using Fluoromount-G (Southern Biotech).

    Article Title: Social transmission of valence-linked new knowledge without firsthand experience in mice
    Article Snippet: Brains were removed and post-fixed in 4 % PFA in PBS at 4 ° C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm. .. For immunohistochemistry (IHC), tissue sections were incubated in 0.3 % Triton-X/PBS (PBS-T) with 10 % normal goat serum (NGS) for 1 h. Primary antibodies were then added to PBS-T with 10 % NGS and tissue sections were incubated overnight at 4 ° C. Primary antibodies used for immunostaining were as follows: guinea pig anti-NeuN (1:1000, Millipore Sigma), rabbit anti-GFP (1:2000, Thermo Fisher Scientific) and rat anti-mCherry (1:2000, Invitrogen). .. After washing with PBS (3 x 5 min), tissue sections were subsequently incubated with AlexaFluor 488, AlexaFluor 546, or AlexaFluor 555 conjugated secondary antibodies (1/500, Thermo Fisher Scientific) in PBS with 10 % NGS for 2 h at R.T. Tissue sections were then incubated in PBS-T with DAPI (1:1000, Thermo Fisher Scientific) for 20 min, and then washed with PBS-T (3 x 10 min) at R.T., and mounted in VECTASHIELD medium (Vector Laboratories) on glass slides.

    Blocking Assay:

    Article Title: Cells and circuits for amygdala neuroplasticity in the transition to chronic pain.
    Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween 20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. The slices were incubated in rat anti-mCherry (1:125, M11217, Invitrogen) and rabbit anti-b-gal (1:500, ab986, Millipore Sigma) primary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA overnight at 4 C. Slices were then rinsed in PBS and incubated in goat anti-rat Cy3 (1:250, A10522, Invitrogen) and Alexa Fluor 647-conjugated goat anti-rabbit (1:500, A21244, Invitrogen) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 h at room temperature. .. Sections were then rinsed in PBS, mounted on positively charged glass slides, air-dried and coverslips were placed using Fluoromount-G (Southern Biotech).

    Article Title: Aberrant TSC-Rheb axis in Oxytocin receptor+ cells mediate stress-induced anxiety
    Article Snippet: The PFA is then replaced with 30% sucrose solution for another 24 h. 40 μm free-floating coronal brain sections containing PFC were collected using Leica vibratome (VT1000s) and stored in 1X PBS containing 0.05% sodium azide at 4°C. .. After blocking in 5% normal goat serum in 0.1 M PBS with 0.1% Triton X-100, brain sections were probed overnight with primary antibodies [chicken anti-EGFP (Abcam #ab13970 ) , rabbit anti-EGFP (Thermo Fisher #G10362 ) , rabbit anti-p-eIF2α S51 (Cell Signaling #9721), mouse anti-NeuN (Millipore Sigma #MAB377), rat anti-mCherry (Thermo Fisher #M11217), rabbit anti-p-S6 (240/244) (Cell Signaling #5364S), mouse anti-p-S6 (S235/6) (Cell Signaling # 62016S), rabbit anti-RHEB (Fisher #PIPA520129) and rabbit anti-TSC2 (Sigma #HPA030409). .. After washing three times in 0.1M PBS, brain sections were incubated with Alexa Fluor conjugated secondary antibodies 1:200 (Thermo Fisher #A-11001, #A32931, #A32733, #A48255, #A48254, #A32740) in blocking buffer for 1.5h at RT and mounted using Prolong Gold antifade mountant with or without DAPI (Fisher # P36931, # P36930).

    Immunostaining:

    Article Title: Cardiomyocyte maturation promoter
    Article Snippet: .. On 8th culture day, the cells were fixed with paraformaldehyde (Wako Pure Chemical Corporation, 163-20145), and subjecting to immunostaining using rat anti-mCherry (Invitrogen, M11217) as a primary antibody and goat anti-rat IgG Alexa 647 (Invitrogen, A-21247) as a secondary antibody. .. The expression level of Alexa 647 was measured by HCS (high contents screening) system (Perkin Elmer/OperaPhenix High Contents Imaging System) (shooting mode; non-confocal, objective lens; 10×air NAO.3).

    Article Title: Social transmission of valence-linked new knowledge without firsthand experience in mice
    Article Snippet: Brains were removed and post-fixed in 4 % PFA in PBS at 4 ° C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm. .. For immunohistochemistry (IHC), tissue sections were incubated in 0.3 % Triton-X/PBS (PBS-T) with 10 % normal goat serum (NGS) for 1 h. Primary antibodies were then added to PBS-T with 10 % NGS and tissue sections were incubated overnight at 4 ° C. Primary antibodies used for immunostaining were as follows: guinea pig anti-NeuN (1:1000, Millipore Sigma), rabbit anti-GFP (1:2000, Thermo Fisher Scientific) and rat anti-mCherry (1:2000, Invitrogen). .. After washing with PBS (3 x 5 min), tissue sections were subsequently incubated with AlexaFluor 488, AlexaFluor 546, or AlexaFluor 555 conjugated secondary antibodies (1/500, Thermo Fisher Scientific) in PBS with 10 % NGS for 2 h at R.T. Tissue sections were then incubated in PBS-T with DAPI (1:1000, Thermo Fisher Scientific) for 20 min, and then washed with PBS-T (3 x 10 min) at R.T., and mounted in VECTASHIELD medium (Vector Laboratories) on glass slides.

    Immunohistochemistry:

    Article Title: Social transmission of valence-linked new knowledge without firsthand experience in mice
    Article Snippet: Brains were removed and post-fixed in 4 % PFA in PBS at 4 ° C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm. .. For immunohistochemistry (IHC), tissue sections were incubated in 0.3 % Triton-X/PBS (PBS-T) with 10 % normal goat serum (NGS) for 1 h. Primary antibodies were then added to PBS-T with 10 % NGS and tissue sections were incubated overnight at 4 ° C. Primary antibodies used for immunostaining were as follows: guinea pig anti-NeuN (1:1000, Millipore Sigma), rabbit anti-GFP (1:2000, Thermo Fisher Scientific) and rat anti-mCherry (1:2000, Invitrogen). .. After washing with PBS (3 x 5 min), tissue sections were subsequently incubated with AlexaFluor 488, AlexaFluor 546, or AlexaFluor 555 conjugated secondary antibodies (1/500, Thermo Fisher Scientific) in PBS with 10 % NGS for 2 h at R.T. Tissue sections were then incubated in PBS-T with DAPI (1:1000, Thermo Fisher Scientific) for 20 min, and then washed with PBS-T (3 x 10 min) at R.T., and mounted in VECTASHIELD medium (Vector Laboratories) on glass slides.



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